Abstract 37

THE EFFECT OF AUTOLOGOUS GROWTH FACTORS ON THE PROLIFERATION OF OSTEOBLAST-LIKE CELLS

Chen J.B., Yu Y., Walsh W.R.

Surgical & Orthopaedic Research Labs, UNSW, POW Hospital, Sydney, NSW 2031, Australia.

Autologous growth factors (AGF) derived from platelets may be of great therapeutic benefits on bone formation. More studies are needed for published conflicting results. This study was to investigate the short-term effect of AGF on osteoblast-like cells. Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) was prepared using Interpore Cross ACCESSTM system from 120ml of whole blood collected from 10 volunteers. AGF was released by mixing PRP or PPP with thrombin (0.5unit/ml). Saos-2, and U2OS cells were maintained in media with 10% FBS. The cells were stimulated with 0% & 10% of FBS, 4%, 0.8%, 0.16% of PRP and PPP for 48hours after starving 12hours. The cell proliferation rate was measured with MTT assay and compared using one-way ANOVA.

The platelet count of PRP was 5.7 times that of whole blood. The effects of AGF on cell proliferation were listed as following (OD,.÷±S): U2OS cells: 0.185±0.004, 0.073±0.009; 0.185±0.012, 0.181±0.018, 0.086±0.006; 0.137±0.018, 0.098±0.010, 0.060±0.003; Saos-2 cells: 0.054±0.006, 0.037±0.006; 0.073±0.002, 0.056±0.005, 0.043±0.005; 0.062±0.009, 0.049±0.006, 0.037±0.006 for 10% & 0% of FBS; 4%, 0.8%, and 0.16% of PRP and PPP. PRP significantly increased proliferation of U2OS cells compared with PPP, while Saos-2 cells did not.

The distinct effect of AGF on proliferation of U2OS and Saos-2 cell lines was noted. It should be careful to explore the effects of growth factors on different cells.

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