Abstract 14

HEALING BONE WITH THE ADIPOSE DERIVED STROMAL STEM CELLS-THE HISTOMORPHOMETRY OF THE OVINE DEFECT MODEL.

Niechoda, B., Yu, Y., Chen, J.B., Walsh, W.R.

Orthopaedic Research Laboratories, Prince of Wales Hospital, University of New South Wales, Sydney, Australia

The objective of this study was to examine the potential of culture expanded, adipose tissue derived stromal stem cell population to heal a critical size confined cancellous bone defect. It has been demonstrated recently, that the adipose-derived stem cells (ADSCs) are capable of osteogenic differentiation under appropriate conditions in vitro.(P.Zuk et al, Tissue Engineering 2001, 7(2).

Bone marrow aspirates and subcutaneous adipose tissue were harvested from 42 adult wethers. The population of stromal cells was derived from both tissues. Populations of bone marrow cells and adipose stromal cells were expanded in culture and stimulated with osteogenic medium for 7 days. Cultured cell populations were harvested, mixed with a hydroxyapatite carrier (Pro-Osteon 200R) and deposited into bilateral medial femoral condyle confined cancellous defect.

7 groups was examined: Bone graft+ ADSCs, Bone graft+ BMSCs, Carrier + ADSCs, Carrier+ BMSCs, Bone graft, Carrier, Empty defect.

2 weeks, 4 weeks and 8 weeks time-points were examined.

Histological staining's: H&E and Masson's were performed and quantified statistically. Immunohistochemical staining for BMP4 and BMP7 and their downstream regulators: Smad4 and CBFA1were evaluated in the defect area and graded in a blind fashion by two trained observers.

There was a progressive and time dependant increase in woven bone formation in the defects treated with ADSCs across all time points. The amount of bone formed in this group was comparable with the amount formed by the use of BMSCs.

The results of this study support the hypothesis that seeding porous hydroxyappatite with ADSCs does enhance bone formation and defect healing.

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